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control aav6 gfp  (Vector Biolabs)


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    Vector Biolabs control aav6 gfp
    Control Aav6 Gfp, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav6+gfp/pmc10208836__mmc2-270-15-23?v=Vector+Biolabs
    Average 95 stars, based on 12 article reviews
    control aav6 gfp - by Bioz Stars, 2026-07
    95/100 stars

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    AAV6 shows high infection efficiency and specificity in lung epithelial cells (A) Schematic illustration of IT injection of AAV-GFPs followed by lung harvest at day14 for fluorescence-activated cell sorting (FACS) and immunofluorescence (IF) staining analysis. (B) The proportion of GFP + cells in live cells in AAV5-GFP-, AAV6-GFP-, and AAV9-GFP-infected lungs by flow cytometry analysis. n = 4 mice per group. (C) The mean GFP intensity in AAV5-GFP-, AAV6-GFP-, and AAV9-GFP-infected lungs by flow cytometry analysis, normalized by that in AAV9 group. n = 4 mice per group. (D) Representative images of GFP immunofluorescence in AAV5-GFP-, AAV6-GFP-, and AAV9-GFP-infected lung sections. Scale bar, 200 μm. (E) The proportion of GFP + cells in different cell types by flow cytometry analysis. n = 4 mice per group. (F and G) Representative images of IF staining of SPC (AT2 cells), RAGE (AT1 cells), CC10 (club cells), and acetylated-tubulin (ciliated cells) in AAV6-GFP infected lung sections. Scale bar, 40 μm. (B, C, and E) Mean ± SEM and p values were analyzed by one-way ANOVA. Data are representative of three independent experiments.

    Journal: Molecular Therapy

    Article Title: CasRx-based Wnt activation promotes alveolar regeneration while ameliorating pulmonary fibrosis in a mouse model of lung injury

    doi: 10.1016/j.ymthe.2024.09.008

    Figure Lengend Snippet: AAV6 shows high infection efficiency and specificity in lung epithelial cells (A) Schematic illustration of IT injection of AAV-GFPs followed by lung harvest at day14 for fluorescence-activated cell sorting (FACS) and immunofluorescence (IF) staining analysis. (B) The proportion of GFP + cells in live cells in AAV5-GFP-, AAV6-GFP-, and AAV9-GFP-infected lungs by flow cytometry analysis. n = 4 mice per group. (C) The mean GFP intensity in AAV5-GFP-, AAV6-GFP-, and AAV9-GFP-infected lungs by flow cytometry analysis, normalized by that in AAV9 group. n = 4 mice per group. (D) Representative images of GFP immunofluorescence in AAV5-GFP-, AAV6-GFP-, and AAV9-GFP-infected lung sections. Scale bar, 200 μm. (E) The proportion of GFP + cells in different cell types by flow cytometry analysis. n = 4 mice per group. (F and G) Representative images of IF staining of SPC (AT2 cells), RAGE (AT1 cells), CC10 (club cells), and acetylated-tubulin (ciliated cells) in AAV6-GFP infected lung sections. Scale bar, 40 μm. (B, C, and E) Mean ± SEM and p values were analyzed by one-way ANOVA. Data are representative of three independent experiments.

    Article Snippet: AAV5-GFP, AAV6-GFP and AAV9-GFP were purchased from PackGene Biotech.

    Techniques: Infection, Injection, Fluorescence, FACS, Immunofluorescence, Staining, Flow Cytometry